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    TaKaRa primescripttm rt reagent kit takara bioscience cat
    Primescripttm Rt Reagent Kit Takara Bioscience Cat, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 88323 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescripttm+rt+reagent+kit+takara+bioscience+cat/PrimeScript+RT+Reagent+Kit/pm37402372-562-13-17
    Average 99 stars, based on 88323 article reviews
    primescripttm rt reagent kit takara bioscience cat - by Bioz Stars, 2026-09
    99/100 stars

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    Western Blot:

    Article Title: Hepatic soluble epoxide hydrolase activity regulates cerebral Aβ metabolism and the pathogenesis of Alzheimer's disease in mice.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 14,15-DHET-d11 Cayman Chemical Cat#10008040 11,12-DHET-d11 Cayman Chemical Cat#10007975 11,12-EET-d11 Cayman Chemical Cat#10006413 5,6-EET Cayman Chemical Cat#50211; CAS: 87173-80-6 8,9-EET Cayman Chemical Cat#50351; CAS: 81246-85-7 11,12-EET Cayman Chemical Cat#50511; CAS: 123931-40-8 14,15-EET Cayman Chemical Cat#50651; CAS: 197508-62-6 5,6-DHET Cayman Chemical Cat#51211; CAS: 213382-49-1 8,9-DHET Cayman Chemical Cat#51351; CAS: 192461-96-4 11,12-DHET Cayman Chemical Cat#51511; CAS: 192461-95-3 14,15-DHET Cayman Chemical Cat#51651; CAS: 77667-09-5 Amyloid b-Protein (1-42), Ab42 BACHEM Cat#4014447; CAS: 107761-42-2 Amyloid b-Protein (1-40), Ab40 BACHEM Cat#4014442; CAS: 131438-79-4 DNase I Roche Cat#10104159001; CAS: 9003-98-9 Trypsin (1mM EDTA) Millipore Cat#SM-2003-C; CAS: 9002-07-7 RNAiso Plus Takara Bioscience Cat#9109 Sulfo-EGS Thermo Fisher Scientific Cat#21566 4,6-diamidino-2-phenylindole (DAPI) Vector Laboratories Cat#H-1200-10 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) Sigma-Aldrich Cat#325244; CAS: 920-66-1 Thioflavin S (ThS) Sigma-Aldrich Cat#T1892; CAS: 1326-12-1 Thioflavin T (ThT) Sigma-Aldrich Cat#T3516; CAS: 2390-54-7 Lipopolysaccharides (LPS) Sigma-Aldrich Cat#L4391; CAS: 93572-42-0 Tamoxifen (TAM) Sigma-Aldrich Cat#T5648; CAS: 10540-29-1 DMSO Sigma-Aldrich Cat#D2650; CAS: 67-68-5 Corn oil Sigma-Aldrich Cat#C8267; CAS: 8001-30-7 Critical commercial assays Human Ab42 ELISA Kit Thermo Fisher Scientific Cat#KHB3441 Human Ab40 ELISA Kit Thermo Fisher Scientific Cat#KHB3481 Mouse ApoE ELISA Kit Abcam Cat#ab215086 BCA Protein Assay Kit Thermo Fisher Scientific Cat#23227 Lipofectamine RNAiMAX transfection reagent Thermo Fisher Scientific Cat#13778100 Bioplex Pro mouse cytokine 23-plex Kit Bio-Rad Cat#M60009RDPD (Continued on next page) e2 Neuron 111, 2847–2862.e1–e10, September 20, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Immobilon ECL ultra western HRP Kit Millipore Cat#WBULS0100 PrimeScriptTM RT Reagent Kit Takara Bioscience CatRR037A SYBR Premix Ex TaqTM Kit Takara Bioscience Cat#RR420A Deposited data Label-free proteomics raw and analyzed data This paper PRIDE: PXD037361 Original Western blots images This paper https://doi.org/10.17632/kbgt58z2nm.1 Experimental models: Organisms/strains Mouse: 53FAD The Jackson Laboratory RRID:MMRRC_034848-JAX Mouse: 33TgAD The Jackson Laboratory RRID:MMRRC_034830-JAX Mouse: Fgfr3-iCreERT2 The Jackson Laboratory RRID:IMSR_JAX:025809 Mouse: Myh6-CreERT2 The Jackson Laboratory RRID:IMSR_JAX:005657 Mouse: Sftpc-CreERT2 The Jackson Laboratory RRID:IMSR_JAX:028054 Mouse: Rosa26 tdTomato The Jackson Laboratory RRID:IMSR_JAX:007909 Mouse: Ephx2loxp/loxp Qin et al.11 and Xiong et al.36 N/A Mouse: Albumin (Alb)-CreERT2 Qin et al.11 N/A Mouse: Ephx2-CreERT2 Xiong et al.36 N/A Mouse: Adult C57BL/6J Beijing Vital River Laboratory Animal Technology Co., Ltd N/A Oligonucleotides siRNA targeting Vti1a sequence, see method details This paper N/A siRNA control sequence (scrambled), see method details This paper N/A Primers for PCR, see Table S5 This paper N/A Software and algorithms Ethovision XT 11.5 Noldus https://www.noldus.com/ethovision-xt NIH Image J National Institutes of Health https://imagej.nih.gov/ij/ Imaris Bitplane http://www.bitplane.com/imaris AlphaEaseFC Alpha Innotech Corporation N/A Prism 9.3.1 GraphPad Software https://www.graphpad.com/ JESS ProteinSimple https://www.bio-techne.com/brands/proteinsimple TraceFinder 4.1 General LC software Thermo Fisher Scientific N/A 7500 Real-Time PCR System platform Thermo Fisher Scientific N/A MaxQuant v1.5.3.30 Thermo Fisher Scientific https://www.maxquant.org/ TBtools Chen et al.59 N/A Other VICTORTMX3 multilabel reader PerkinElmer N/A Freezing microtome Leica N/A Fluorescent microscopy Nikon Instruments Inc. N/A SpeedVac Eppendorf N/A Osmotic pump Alzet Cat#Model 1007D ..

    Sequencing:

    Article Title: Hepatic soluble epoxide hydrolase activity regulates cerebral Aβ metabolism and the pathogenesis of Alzheimer's disease in mice.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 14,15-DHET-d11 Cayman Chemical Cat#10008040 11,12-DHET-d11 Cayman Chemical Cat#10007975 11,12-EET-d11 Cayman Chemical Cat#10006413 5,6-EET Cayman Chemical Cat#50211; CAS: 87173-80-6 8,9-EET Cayman Chemical Cat#50351; CAS: 81246-85-7 11,12-EET Cayman Chemical Cat#50511; CAS: 123931-40-8 14,15-EET Cayman Chemical Cat#50651; CAS: 197508-62-6 5,6-DHET Cayman Chemical Cat#51211; CAS: 213382-49-1 8,9-DHET Cayman Chemical Cat#51351; CAS: 192461-96-4 11,12-DHET Cayman Chemical Cat#51511; CAS: 192461-95-3 14,15-DHET Cayman Chemical Cat#51651; CAS: 77667-09-5 Amyloid b-Protein (1-42), Ab42 BACHEM Cat#4014447; CAS: 107761-42-2 Amyloid b-Protein (1-40), Ab40 BACHEM Cat#4014442; CAS: 131438-79-4 DNase I Roche Cat#10104159001; CAS: 9003-98-9 Trypsin (1mM EDTA) Millipore Cat#SM-2003-C; CAS: 9002-07-7 RNAiso Plus Takara Bioscience Cat#9109 Sulfo-EGS Thermo Fisher Scientific Cat#21566 4,6-diamidino-2-phenylindole (DAPI) Vector Laboratories Cat#H-1200-10 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) Sigma-Aldrich Cat#325244; CAS: 920-66-1 Thioflavin S (ThS) Sigma-Aldrich Cat#T1892; CAS: 1326-12-1 Thioflavin T (ThT) Sigma-Aldrich Cat#T3516; CAS: 2390-54-7 Lipopolysaccharides (LPS) Sigma-Aldrich Cat#L4391; CAS: 93572-42-0 Tamoxifen (TAM) Sigma-Aldrich Cat#T5648; CAS: 10540-29-1 DMSO Sigma-Aldrich Cat#D2650; CAS: 67-68-5 Corn oil Sigma-Aldrich Cat#C8267; CAS: 8001-30-7 Critical commercial assays Human Ab42 ELISA Kit Thermo Fisher Scientific Cat#KHB3441 Human Ab40 ELISA Kit Thermo Fisher Scientific Cat#KHB3481 Mouse ApoE ELISA Kit Abcam Cat#ab215086 BCA Protein Assay Kit Thermo Fisher Scientific Cat#23227 Lipofectamine RNAiMAX transfection reagent Thermo Fisher Scientific Cat#13778100 Bioplex Pro mouse cytokine 23-plex Kit Bio-Rad Cat#M60009RDPD (Continued on next page) e2 Neuron 111, 2847–2862.e1–e10, September 20, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Immobilon ECL ultra western HRP Kit Millipore Cat#WBULS0100 PrimeScriptTM RT Reagent Kit Takara Bioscience CatRR037A SYBR Premix Ex TaqTM Kit Takara Bioscience Cat#RR420A Deposited data Label-free proteomics raw and analyzed data This paper PRIDE: PXD037361 Original Western blots images This paper https://doi.org/10.17632/kbgt58z2nm.1 Experimental models: Organisms/strains Mouse: 53FAD The Jackson Laboratory RRID:MMRRC_034848-JAX Mouse: 33TgAD The Jackson Laboratory RRID:MMRRC_034830-JAX Mouse: Fgfr3-iCreERT2 The Jackson Laboratory RRID:IMSR_JAX:025809 Mouse: Myh6-CreERT2 The Jackson Laboratory RRID:IMSR_JAX:005657 Mouse: Sftpc-CreERT2 The Jackson Laboratory RRID:IMSR_JAX:028054 Mouse: Rosa26 tdTomato The Jackson Laboratory RRID:IMSR_JAX:007909 Mouse: Ephx2loxp/loxp Qin et al.11 and Xiong et al.36 N/A Mouse: Albumin (Alb)-CreERT2 Qin et al.11 N/A Mouse: Ephx2-CreERT2 Xiong et al.36 N/A Mouse: Adult C57BL/6J Beijing Vital River Laboratory Animal Technology Co., Ltd N/A Oligonucleotides siRNA targeting Vti1a sequence, see method details This paper N/A siRNA control sequence (scrambled), see method details This paper N/A Primers for PCR, see Table S5 This paper N/A Software and algorithms Ethovision XT 11.5 Noldus https://www.noldus.com/ethovision-xt NIH Image J National Institutes of Health https://imagej.nih.gov/ij/ Imaris Bitplane http://www.bitplane.com/imaris AlphaEaseFC Alpha Innotech Corporation N/A Prism 9.3.1 GraphPad Software https://www.graphpad.com/ JESS ProteinSimple https://www.bio-techne.com/brands/proteinsimple TraceFinder 4.1 General LC software Thermo Fisher Scientific N/A 7500 Real-Time PCR System platform Thermo Fisher Scientific N/A MaxQuant v1.5.3.30 Thermo Fisher Scientific https://www.maxquant.org/ TBtools Chen et al.59 N/A Other VICTORTMX3 multilabel reader PerkinElmer N/A Freezing microtome Leica N/A Fluorescent microscopy Nikon Instruments Inc. N/A SpeedVac Eppendorf N/A Osmotic pump Alzet Cat#Model 1007D ..

    Control:

    Article Title: Hepatic soluble epoxide hydrolase activity regulates cerebral Aβ metabolism and the pathogenesis of Alzheimer's disease in mice.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 14,15-DHET-d11 Cayman Chemical Cat#10008040 11,12-DHET-d11 Cayman Chemical Cat#10007975 11,12-EET-d11 Cayman Chemical Cat#10006413 5,6-EET Cayman Chemical Cat#50211; CAS: 87173-80-6 8,9-EET Cayman Chemical Cat#50351; CAS: 81246-85-7 11,12-EET Cayman Chemical Cat#50511; CAS: 123931-40-8 14,15-EET Cayman Chemical Cat#50651; CAS: 197508-62-6 5,6-DHET Cayman Chemical Cat#51211; CAS: 213382-49-1 8,9-DHET Cayman Chemical Cat#51351; CAS: 192461-96-4 11,12-DHET Cayman Chemical Cat#51511; CAS: 192461-95-3 14,15-DHET Cayman Chemical Cat#51651; CAS: 77667-09-5 Amyloid b-Protein (1-42), Ab42 BACHEM Cat#4014447; CAS: 107761-42-2 Amyloid b-Protein (1-40), Ab40 BACHEM Cat#4014442; CAS: 131438-79-4 DNase I Roche Cat#10104159001; CAS: 9003-98-9 Trypsin (1mM EDTA) Millipore Cat#SM-2003-C; CAS: 9002-07-7 RNAiso Plus Takara Bioscience Cat#9109 Sulfo-EGS Thermo Fisher Scientific Cat#21566 4,6-diamidino-2-phenylindole (DAPI) Vector Laboratories Cat#H-1200-10 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) Sigma-Aldrich Cat#325244; CAS: 920-66-1 Thioflavin S (ThS) Sigma-Aldrich Cat#T1892; CAS: 1326-12-1 Thioflavin T (ThT) Sigma-Aldrich Cat#T3516; CAS: 2390-54-7 Lipopolysaccharides (LPS) Sigma-Aldrich Cat#L4391; CAS: 93572-42-0 Tamoxifen (TAM) Sigma-Aldrich Cat#T5648; CAS: 10540-29-1 DMSO Sigma-Aldrich Cat#D2650; CAS: 67-68-5 Corn oil Sigma-Aldrich Cat#C8267; CAS: 8001-30-7 Critical commercial assays Human Ab42 ELISA Kit Thermo Fisher Scientific Cat#KHB3441 Human Ab40 ELISA Kit Thermo Fisher Scientific Cat#KHB3481 Mouse ApoE ELISA Kit Abcam Cat#ab215086 BCA Protein Assay Kit Thermo Fisher Scientific Cat#23227 Lipofectamine RNAiMAX transfection reagent Thermo Fisher Scientific Cat#13778100 Bioplex Pro mouse cytokine 23-plex Kit Bio-Rad Cat#M60009RDPD (Continued on next page) e2 Neuron 111, 2847–2862.e1–e10, September 20, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Immobilon ECL ultra western HRP Kit Millipore Cat#WBULS0100 PrimeScriptTM RT Reagent Kit Takara Bioscience CatRR037A SYBR Premix Ex TaqTM Kit Takara Bioscience Cat#RR420A Deposited data Label-free proteomics raw and analyzed data This paper PRIDE: PXD037361 Original Western blots images This paper https://doi.org/10.17632/kbgt58z2nm.1 Experimental models: Organisms/strains Mouse: 53FAD The Jackson Laboratory RRID:MMRRC_034848-JAX Mouse: 33TgAD The Jackson Laboratory RRID:MMRRC_034830-JAX Mouse: Fgfr3-iCreERT2 The Jackson Laboratory RRID:IMSR_JAX:025809 Mouse: Myh6-CreERT2 The Jackson Laboratory RRID:IMSR_JAX:005657 Mouse: Sftpc-CreERT2 The Jackson Laboratory RRID:IMSR_JAX:028054 Mouse: Rosa26 tdTomato The Jackson Laboratory RRID:IMSR_JAX:007909 Mouse: Ephx2loxp/loxp Qin et al.11 and Xiong et al.36 N/A Mouse: Albumin (Alb)-CreERT2 Qin et al.11 N/A Mouse: Ephx2-CreERT2 Xiong et al.36 N/A Mouse: Adult C57BL/6J Beijing Vital River Laboratory Animal Technology Co., Ltd N/A Oligonucleotides siRNA targeting Vti1a sequence, see method details This paper N/A siRNA control sequence (scrambled), see method details This paper N/A Primers for PCR, see Table S5 This paper N/A Software and algorithms Ethovision XT 11.5 Noldus https://www.noldus.com/ethovision-xt NIH Image J National Institutes of Health https://imagej.nih.gov/ij/ Imaris Bitplane http://www.bitplane.com/imaris AlphaEaseFC Alpha Innotech Corporation N/A Prism 9.3.1 GraphPad Software https://www.graphpad.com/ JESS ProteinSimple https://www.bio-techne.com/brands/proteinsimple TraceFinder 4.1 General LC software Thermo Fisher Scientific N/A 7500 Real-Time PCR System platform Thermo Fisher Scientific N/A MaxQuant v1.5.3.30 Thermo Fisher Scientific https://www.maxquant.org/ TBtools Chen et al.59 N/A Other VICTORTMX3 multilabel reader PerkinElmer N/A Freezing microtome Leica N/A Fluorescent microscopy Nikon Instruments Inc. N/A SpeedVac Eppendorf N/A Osmotic pump Alzet Cat#Model 1007D ..

    Polymerase Chain Reaction:

    Article Title: Hepatic soluble epoxide hydrolase activity regulates cerebral Aβ metabolism and the pathogenesis of Alzheimer's disease in mice.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 14,15-DHET-d11 Cayman Chemical Cat#10008040 11,12-DHET-d11 Cayman Chemical Cat#10007975 11,12-EET-d11 Cayman Chemical Cat#10006413 5,6-EET Cayman Chemical Cat#50211; CAS: 87173-80-6 8,9-EET Cayman Chemical Cat#50351; CAS: 81246-85-7 11,12-EET Cayman Chemical Cat#50511; CAS: 123931-40-8 14,15-EET Cayman Chemical Cat#50651; CAS: 197508-62-6 5,6-DHET Cayman Chemical Cat#51211; CAS: 213382-49-1 8,9-DHET Cayman Chemical Cat#51351; CAS: 192461-96-4 11,12-DHET Cayman Chemical Cat#51511; CAS: 192461-95-3 14,15-DHET Cayman Chemical Cat#51651; CAS: 77667-09-5 Amyloid b-Protein (1-42), Ab42 BACHEM Cat#4014447; CAS: 107761-42-2 Amyloid b-Protein (1-40), Ab40 BACHEM Cat#4014442; CAS: 131438-79-4 DNase I Roche Cat#10104159001; CAS: 9003-98-9 Trypsin (1mM EDTA) Millipore Cat#SM-2003-C; CAS: 9002-07-7 RNAiso Plus Takara Bioscience Cat#9109 Sulfo-EGS Thermo Fisher Scientific Cat#21566 4,6-diamidino-2-phenylindole (DAPI) Vector Laboratories Cat#H-1200-10 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) Sigma-Aldrich Cat#325244; CAS: 920-66-1 Thioflavin S (ThS) Sigma-Aldrich Cat#T1892; CAS: 1326-12-1 Thioflavin T (ThT) Sigma-Aldrich Cat#T3516; CAS: 2390-54-7 Lipopolysaccharides (LPS) Sigma-Aldrich Cat#L4391; CAS: 93572-42-0 Tamoxifen (TAM) Sigma-Aldrich Cat#T5648; CAS: 10540-29-1 DMSO Sigma-Aldrich Cat#D2650; CAS: 67-68-5 Corn oil Sigma-Aldrich Cat#C8267; CAS: 8001-30-7 Critical commercial assays Human Ab42 ELISA Kit Thermo Fisher Scientific Cat#KHB3441 Human Ab40 ELISA Kit Thermo Fisher Scientific Cat#KHB3481 Mouse ApoE ELISA Kit Abcam Cat#ab215086 BCA Protein Assay Kit Thermo Fisher Scientific Cat#23227 Lipofectamine RNAiMAX transfection reagent Thermo Fisher Scientific Cat#13778100 Bioplex Pro mouse cytokine 23-plex Kit Bio-Rad Cat#M60009RDPD (Continued on next page) e2 Neuron 111, 2847–2862.e1–e10, September 20, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Immobilon ECL ultra western HRP Kit Millipore Cat#WBULS0100 PrimeScriptTM RT Reagent Kit Takara Bioscience CatRR037A SYBR Premix Ex TaqTM Kit Takara Bioscience Cat#RR420A Deposited data Label-free proteomics raw and analyzed data This paper PRIDE: PXD037361 Original Western blots images This paper https://doi.org/10.17632/kbgt58z2nm.1 Experimental models: Organisms/strains Mouse: 53FAD The Jackson Laboratory RRID:MMRRC_034848-JAX Mouse: 33TgAD The Jackson Laboratory RRID:MMRRC_034830-JAX Mouse: Fgfr3-iCreERT2 The Jackson Laboratory RRID:IMSR_JAX:025809 Mouse: Myh6-CreERT2 The Jackson Laboratory RRID:IMSR_JAX:005657 Mouse: Sftpc-CreERT2 The Jackson Laboratory RRID:IMSR_JAX:028054 Mouse: Rosa26 tdTomato The Jackson Laboratory RRID:IMSR_JAX:007909 Mouse: Ephx2loxp/loxp Qin et al.11 and Xiong et al.36 N/A Mouse: Albumin (Alb)-CreERT2 Qin et al.11 N/A Mouse: Ephx2-CreERT2 Xiong et al.36 N/A Mouse: Adult C57BL/6J Beijing Vital River Laboratory Animal Technology Co., Ltd N/A Oligonucleotides siRNA targeting Vti1a sequence, see method details This paper N/A siRNA control sequence (scrambled), see method details This paper N/A Primers for PCR, see Table S5 This paper N/A Software and algorithms Ethovision XT 11.5 Noldus https://www.noldus.com/ethovision-xt NIH Image J National Institutes of Health https://imagej.nih.gov/ij/ Imaris Bitplane http://www.bitplane.com/imaris AlphaEaseFC Alpha Innotech Corporation N/A Prism 9.3.1 GraphPad Software https://www.graphpad.com/ JESS ProteinSimple https://www.bio-techne.com/brands/proteinsimple TraceFinder 4.1 General LC software Thermo Fisher Scientific N/A 7500 Real-Time PCR System platform Thermo Fisher Scientific N/A MaxQuant v1.5.3.30 Thermo Fisher Scientific https://www.maxquant.org/ TBtools Chen et al.59 N/A Other VICTORTMX3 multilabel reader PerkinElmer N/A Freezing microtome Leica N/A Fluorescent microscopy Nikon Instruments Inc. N/A SpeedVac Eppendorf N/A Osmotic pump Alzet Cat#Model 1007D ..

    Software:

    Article Title: Hepatic soluble epoxide hydrolase activity regulates cerebral Aβ metabolism and the pathogenesis of Alzheimer's disease in mice.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 14,15-DHET-d11 Cayman Chemical Cat#10008040 11,12-DHET-d11 Cayman Chemical Cat#10007975 11,12-EET-d11 Cayman Chemical Cat#10006413 5,6-EET Cayman Chemical Cat#50211; CAS: 87173-80-6 8,9-EET Cayman Chemical Cat#50351; CAS: 81246-85-7 11,12-EET Cayman Chemical Cat#50511; CAS: 123931-40-8 14,15-EET Cayman Chemical Cat#50651; CAS: 197508-62-6 5,6-DHET Cayman Chemical Cat#51211; CAS: 213382-49-1 8,9-DHET Cayman Chemical Cat#51351; CAS: 192461-96-4 11,12-DHET Cayman Chemical Cat#51511; CAS: 192461-95-3 14,15-DHET Cayman Chemical Cat#51651; CAS: 77667-09-5 Amyloid b-Protein (1-42), Ab42 BACHEM Cat#4014447; CAS: 107761-42-2 Amyloid b-Protein (1-40), Ab40 BACHEM Cat#4014442; CAS: 131438-79-4 DNase I Roche Cat#10104159001; CAS: 9003-98-9 Trypsin (1mM EDTA) Millipore Cat#SM-2003-C; CAS: 9002-07-7 RNAiso Plus Takara Bioscience Cat#9109 Sulfo-EGS Thermo Fisher Scientific Cat#21566 4,6-diamidino-2-phenylindole (DAPI) Vector Laboratories Cat#H-1200-10 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) Sigma-Aldrich Cat#325244; CAS: 920-66-1 Thioflavin S (ThS) Sigma-Aldrich Cat#T1892; CAS: 1326-12-1 Thioflavin T (ThT) Sigma-Aldrich Cat#T3516; CAS: 2390-54-7 Lipopolysaccharides (LPS) Sigma-Aldrich Cat#L4391; CAS: 93572-42-0 Tamoxifen (TAM) Sigma-Aldrich Cat#T5648; CAS: 10540-29-1 DMSO Sigma-Aldrich Cat#D2650; CAS: 67-68-5 Corn oil Sigma-Aldrich Cat#C8267; CAS: 8001-30-7 Critical commercial assays Human Ab42 ELISA Kit Thermo Fisher Scientific Cat#KHB3441 Human Ab40 ELISA Kit Thermo Fisher Scientific Cat#KHB3481 Mouse ApoE ELISA Kit Abcam Cat#ab215086 BCA Protein Assay Kit Thermo Fisher Scientific Cat#23227 Lipofectamine RNAiMAX transfection reagent Thermo Fisher Scientific Cat#13778100 Bioplex Pro mouse cytokine 23-plex Kit Bio-Rad Cat#M60009RDPD (Continued on next page) e2 Neuron 111, 2847–2862.e1–e10, September 20, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Immobilon ECL ultra western HRP Kit Millipore Cat#WBULS0100 PrimeScriptTM RT Reagent Kit Takara Bioscience CatRR037A SYBR Premix Ex TaqTM Kit Takara Bioscience Cat#RR420A Deposited data Label-free proteomics raw and analyzed data This paper PRIDE: PXD037361 Original Western blots images This paper https://doi.org/10.17632/kbgt58z2nm.1 Experimental models: Organisms/strains Mouse: 53FAD The Jackson Laboratory RRID:MMRRC_034848-JAX Mouse: 33TgAD The Jackson Laboratory RRID:MMRRC_034830-JAX Mouse: Fgfr3-iCreERT2 The Jackson Laboratory RRID:IMSR_JAX:025809 Mouse: Myh6-CreERT2 The Jackson Laboratory RRID:IMSR_JAX:005657 Mouse: Sftpc-CreERT2 The Jackson Laboratory RRID:IMSR_JAX:028054 Mouse: Rosa26 tdTomato The Jackson Laboratory RRID:IMSR_JAX:007909 Mouse: Ephx2loxp/loxp Qin et al.11 and Xiong et al.36 N/A Mouse: Albumin (Alb)-CreERT2 Qin et al.11 N/A Mouse: Ephx2-CreERT2 Xiong et al.36 N/A Mouse: Adult C57BL/6J Beijing Vital River Laboratory Animal Technology Co., Ltd N/A Oligonucleotides siRNA targeting Vti1a sequence, see method details This paper N/A siRNA control sequence (scrambled), see method details This paper N/A Primers for PCR, see Table S5 This paper N/A Software and algorithms Ethovision XT 11.5 Noldus https://www.noldus.com/ethovision-xt NIH Image J National Institutes of Health https://imagej.nih.gov/ij/ Imaris Bitplane http://www.bitplane.com/imaris AlphaEaseFC Alpha Innotech Corporation N/A Prism 9.3.1 GraphPad Software https://www.graphpad.com/ JESS ProteinSimple https://www.bio-techne.com/brands/proteinsimple TraceFinder 4.1 General LC software Thermo Fisher Scientific N/A 7500 Real-Time PCR System platform Thermo Fisher Scientific N/A MaxQuant v1.5.3.30 Thermo Fisher Scientific https://www.maxquant.org/ TBtools Chen et al.59 N/A Other VICTORTMX3 multilabel reader PerkinElmer N/A Freezing microtome Leica N/A Fluorescent microscopy Nikon Instruments Inc. N/A SpeedVac Eppendorf N/A Osmotic pump Alzet Cat#Model 1007D ..

    Real-time Polymerase Chain Reaction:

    Article Title: Hepatic soluble epoxide hydrolase activity regulates cerebral Aβ metabolism and the pathogenesis of Alzheimer's disease in mice.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 14,15-DHET-d11 Cayman Chemical Cat#10008040 11,12-DHET-d11 Cayman Chemical Cat#10007975 11,12-EET-d11 Cayman Chemical Cat#10006413 5,6-EET Cayman Chemical Cat#50211; CAS: 87173-80-6 8,9-EET Cayman Chemical Cat#50351; CAS: 81246-85-7 11,12-EET Cayman Chemical Cat#50511; CAS: 123931-40-8 14,15-EET Cayman Chemical Cat#50651; CAS: 197508-62-6 5,6-DHET Cayman Chemical Cat#51211; CAS: 213382-49-1 8,9-DHET Cayman Chemical Cat#51351; CAS: 192461-96-4 11,12-DHET Cayman Chemical Cat#51511; CAS: 192461-95-3 14,15-DHET Cayman Chemical Cat#51651; CAS: 77667-09-5 Amyloid b-Protein (1-42), Ab42 BACHEM Cat#4014447; CAS: 107761-42-2 Amyloid b-Protein (1-40), Ab40 BACHEM Cat#4014442; CAS: 131438-79-4 DNase I Roche Cat#10104159001; CAS: 9003-98-9 Trypsin (1mM EDTA) Millipore Cat#SM-2003-C; CAS: 9002-07-7 RNAiso Plus Takara Bioscience Cat#9109 Sulfo-EGS Thermo Fisher Scientific Cat#21566 4,6-diamidino-2-phenylindole (DAPI) Vector Laboratories Cat#H-1200-10 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) Sigma-Aldrich Cat#325244; CAS: 920-66-1 Thioflavin S (ThS) Sigma-Aldrich Cat#T1892; CAS: 1326-12-1 Thioflavin T (ThT) Sigma-Aldrich Cat#T3516; CAS: 2390-54-7 Lipopolysaccharides (LPS) Sigma-Aldrich Cat#L4391; CAS: 93572-42-0 Tamoxifen (TAM) Sigma-Aldrich Cat#T5648; CAS: 10540-29-1 DMSO Sigma-Aldrich Cat#D2650; CAS: 67-68-5 Corn oil Sigma-Aldrich Cat#C8267; CAS: 8001-30-7 Critical commercial assays Human Ab42 ELISA Kit Thermo Fisher Scientific Cat#KHB3441 Human Ab40 ELISA Kit Thermo Fisher Scientific Cat#KHB3481 Mouse ApoE ELISA Kit Abcam Cat#ab215086 BCA Protein Assay Kit Thermo Fisher Scientific Cat#23227 Lipofectamine RNAiMAX transfection reagent Thermo Fisher Scientific Cat#13778100 Bioplex Pro mouse cytokine 23-plex Kit Bio-Rad Cat#M60009RDPD (Continued on next page) e2 Neuron 111, 2847–2862.e1–e10, September 20, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Immobilon ECL ultra western HRP Kit Millipore Cat#WBULS0100 PrimeScriptTM RT Reagent Kit Takara Bioscience CatRR037A SYBR Premix Ex TaqTM Kit Takara Bioscience Cat#RR420A Deposited data Label-free proteomics raw and analyzed data This paper PRIDE: PXD037361 Original Western blots images This paper https://doi.org/10.17632/kbgt58z2nm.1 Experimental models: Organisms/strains Mouse: 53FAD The Jackson Laboratory RRID:MMRRC_034848-JAX Mouse: 33TgAD The Jackson Laboratory RRID:MMRRC_034830-JAX Mouse: Fgfr3-iCreERT2 The Jackson Laboratory RRID:IMSR_JAX:025809 Mouse: Myh6-CreERT2 The Jackson Laboratory RRID:IMSR_JAX:005657 Mouse: Sftpc-CreERT2 The Jackson Laboratory RRID:IMSR_JAX:028054 Mouse: Rosa26 tdTomato The Jackson Laboratory RRID:IMSR_JAX:007909 Mouse: Ephx2loxp/loxp Qin et al.11 and Xiong et al.36 N/A Mouse: Albumin (Alb)-CreERT2 Qin et al.11 N/A Mouse: Ephx2-CreERT2 Xiong et al.36 N/A Mouse: Adult C57BL/6J Beijing Vital River Laboratory Animal Technology Co., Ltd N/A Oligonucleotides siRNA targeting Vti1a sequence, see method details This paper N/A siRNA control sequence (scrambled), see method details This paper N/A Primers for PCR, see Table S5 This paper N/A Software and algorithms Ethovision XT 11.5 Noldus https://www.noldus.com/ethovision-xt NIH Image J National Institutes of Health https://imagej.nih.gov/ij/ Imaris Bitplane http://www.bitplane.com/imaris AlphaEaseFC Alpha Innotech Corporation N/A Prism 9.3.1 GraphPad Software https://www.graphpad.com/ JESS ProteinSimple https://www.bio-techne.com/brands/proteinsimple TraceFinder 4.1 General LC software Thermo Fisher Scientific N/A 7500 Real-Time PCR System platform Thermo Fisher Scientific N/A MaxQuant v1.5.3.30 Thermo Fisher Scientific https://www.maxquant.org/ TBtools Chen et al.59 N/A Other VICTORTMX3 multilabel reader PerkinElmer N/A Freezing microtome Leica N/A Fluorescent microscopy Nikon Instruments Inc. N/A SpeedVac Eppendorf N/A Osmotic pump Alzet Cat#Model 1007D ..

    Microscopy:

    Article Title: Hepatic soluble epoxide hydrolase activity regulates cerebral Aβ metabolism and the pathogenesis of Alzheimer's disease in mice.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 14,15-DHET-d11 Cayman Chemical Cat#10008040 11,12-DHET-d11 Cayman Chemical Cat#10007975 11,12-EET-d11 Cayman Chemical Cat#10006413 5,6-EET Cayman Chemical Cat#50211; CAS: 87173-80-6 8,9-EET Cayman Chemical Cat#50351; CAS: 81246-85-7 11,12-EET Cayman Chemical Cat#50511; CAS: 123931-40-8 14,15-EET Cayman Chemical Cat#50651; CAS: 197508-62-6 5,6-DHET Cayman Chemical Cat#51211; CAS: 213382-49-1 8,9-DHET Cayman Chemical Cat#51351; CAS: 192461-96-4 11,12-DHET Cayman Chemical Cat#51511; CAS: 192461-95-3 14,15-DHET Cayman Chemical Cat#51651; CAS: 77667-09-5 Amyloid b-Protein (1-42), Ab42 BACHEM Cat#4014447; CAS: 107761-42-2 Amyloid b-Protein (1-40), Ab40 BACHEM Cat#4014442; CAS: 131438-79-4 DNase I Roche Cat#10104159001; CAS: 9003-98-9 Trypsin (1mM EDTA) Millipore Cat#SM-2003-C; CAS: 9002-07-7 RNAiso Plus Takara Bioscience Cat#9109 Sulfo-EGS Thermo Fisher Scientific Cat#21566 4,6-diamidino-2-phenylindole (DAPI) Vector Laboratories Cat#H-1200-10 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) Sigma-Aldrich Cat#325244; CAS: 920-66-1 Thioflavin S (ThS) Sigma-Aldrich Cat#T1892; CAS: 1326-12-1 Thioflavin T (ThT) Sigma-Aldrich Cat#T3516; CAS: 2390-54-7 Lipopolysaccharides (LPS) Sigma-Aldrich Cat#L4391; CAS: 93572-42-0 Tamoxifen (TAM) Sigma-Aldrich Cat#T5648; CAS: 10540-29-1 DMSO Sigma-Aldrich Cat#D2650; CAS: 67-68-5 Corn oil Sigma-Aldrich Cat#C8267; CAS: 8001-30-7 Critical commercial assays Human Ab42 ELISA Kit Thermo Fisher Scientific Cat#KHB3441 Human Ab40 ELISA Kit Thermo Fisher Scientific Cat#KHB3481 Mouse ApoE ELISA Kit Abcam Cat#ab215086 BCA Protein Assay Kit Thermo Fisher Scientific Cat#23227 Lipofectamine RNAiMAX transfection reagent Thermo Fisher Scientific Cat#13778100 Bioplex Pro mouse cytokine 23-plex Kit Bio-Rad Cat#M60009RDPD (Continued on next page) e2 Neuron 111, 2847–2862.e1–e10, September 20, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Immobilon ECL ultra western HRP Kit Millipore Cat#WBULS0100 PrimeScriptTM RT Reagent Kit Takara Bioscience CatRR037A SYBR Premix Ex TaqTM Kit Takara Bioscience Cat#RR420A Deposited data Label-free proteomics raw and analyzed data This paper PRIDE: PXD037361 Original Western blots images This paper https://doi.org/10.17632/kbgt58z2nm.1 Experimental models: Organisms/strains Mouse: 53FAD The Jackson Laboratory RRID:MMRRC_034848-JAX Mouse: 33TgAD The Jackson Laboratory RRID:MMRRC_034830-JAX Mouse: Fgfr3-iCreERT2 The Jackson Laboratory RRID:IMSR_JAX:025809 Mouse: Myh6-CreERT2 The Jackson Laboratory RRID:IMSR_JAX:005657 Mouse: Sftpc-CreERT2 The Jackson Laboratory RRID:IMSR_JAX:028054 Mouse: Rosa26 tdTomato The Jackson Laboratory RRID:IMSR_JAX:007909 Mouse: Ephx2loxp/loxp Qin et al.11 and Xiong et al.36 N/A Mouse: Albumin (Alb)-CreERT2 Qin et al.11 N/A Mouse: Ephx2-CreERT2 Xiong et al.36 N/A Mouse: Adult C57BL/6J Beijing Vital River Laboratory Animal Technology Co., Ltd N/A Oligonucleotides siRNA targeting Vti1a sequence, see method details This paper N/A siRNA control sequence (scrambled), see method details This paper N/A Primers for PCR, see Table S5 This paper N/A Software and algorithms Ethovision XT 11.5 Noldus https://www.noldus.com/ethovision-xt NIH Image J National Institutes of Health https://imagej.nih.gov/ij/ Imaris Bitplane http://www.bitplane.com/imaris AlphaEaseFC Alpha Innotech Corporation N/A Prism 9.3.1 GraphPad Software https://www.graphpad.com/ JESS ProteinSimple https://www.bio-techne.com/brands/proteinsimple TraceFinder 4.1 General LC software Thermo Fisher Scientific N/A 7500 Real-Time PCR System platform Thermo Fisher Scientific N/A MaxQuant v1.5.3.30 Thermo Fisher Scientific https://www.maxquant.org/ TBtools Chen et al.59 N/A Other VICTORTMX3 multilabel reader PerkinElmer N/A Freezing microtome Leica N/A Fluorescent microscopy Nikon Instruments Inc. N/A SpeedVac Eppendorf N/A Osmotic pump Alzet Cat#Model 1007D ..



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